Journal: PLOS Pathogens

Article Title: Ebola virus disrupts the inner blood-retinal barrier by induction of vascular endothelial growth factor in pericytes

doi: 10.1371/journal.ppat.1011077

Figure Lengend Snippet: (A) Representative cytokines secreted by HREC, HRP and HRA after 48 h of EBO-VLP stimulation. Representative images of three independent experiments are shown. (B) Relative cytokine levels were normalized to the mock group without EBO-VLP administration, which was set as 1. The floating bar plot shows the mean, minimum and maximum levels of each cytokine in three independent experiments. Downregulation of cytokines expression were displayed with negative numbers. (C) Changes in VEGF expression after EBO-VLP administration, as quantified by ELISA. The horizontal dashed line marks the limit of detection of the assay. The results are presented as the means ± standard deviation of three independent experiments. Statistical analysis was performed using Student’s t test. *p < 0.05, **p < 0.01.

Article Snippet: The amount of VEGF in the supernatant was measured by a Human VEGF ELISA Kit (Boster, EK0539).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: PLOS Pathogens

Article Title: Ebola virus disrupts the inner blood-retinal barrier by induction of vascular endothelial growth factor in pericytes

doi: 10.1371/journal.ppat.1011077

Figure Lengend Snippet: (A-B) Integrity of the tri-culture iBRB model after EBO-VLP, EBO-VLP + Avastin and VEGF treatment. Na-F permeability (A) and the TEER values (B) of the iBRB model were examined 48 h after EBO-VLP administration. TEER values were normalized to those of iBRB models themselves before EBO-VLP administration. The box and the whisker present the median ± percentiles (25–75%) and range, respectively. The fold change of permeability compared with iBRB model itself before EBO-VLP administration is presented as the mean ± standard deviation. All values were determined in ten independent experiments. (C) Experimental schematic showing the addition of EBO-VLPs to the iBRB co-culture model of HREC and HRP. (D-E) Integrity of the iBRB co-culture (HRECs and HRPs) model after EBO-VLP, EBO-VLP + Avastin and VEGF treatment. Na-F permeability (D) and the TEER values (E) of the iBRB model were examined after 48 h of treatment. TEER values were normalized to those of iBRB models themselves before EBO-VLP administration. The box and the whisker present the median ± percentiles (25–75%) and range, respectively. The fold change of permeability compared with iBRB model itself before EBO-VLP administration is presented as the mean ± standard deviation. All values were determined in ten independent experiments. (F) Experimental schematic showing the addition of EBO-VLPs to the iBRB co-culture model of HREC and HRA. (G-H) Integrity of the iBRB co-culture (HRECs and HRAs) model after EBO-VLP, EBO-VLP + Avastin, and VEGF treatment. Na-F permeability (G) and the TEER values (H) of the iBRB model were examined 48 h after EBO-VLP administration. All values were determined in ten independent experiments. (I) Changes in VEGF expression after EBO-VLP administration, as quantified by ELISA. Pericytes were transfected with si-VEGF or nontargeting control siRNA, followed by treatment with EBO-VLP for 48 h. The horizontal dashed line marks the limit of detection of the assay. The results are presented as the means ± standard deviation of three independent experiments. (J-K) Integrity of the tri-culture iBRB model after EBO-VLP, si-VEGF + EBO-VLP, nontargeting control siRNA + EBO-VLP, and nontargeting control siRNA treatment. Na-F permeability (J) and the TEER values (K) of the iBRB model were examined 48 h after administration. TEER values were normalized to those of iBRB models themselves before EBO-VLP administration. All values were determined in six independent experiments. Statistical analysis was performed using Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The amount of VEGF in the supernatant was measured by a Human VEGF ELISA Kit (Boster, EK0539).

Techniques: Permeability, Whisker Assay, Standard Deviation, Co-Culture Assay, Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Control

Journal: PLOS Pathogens

Article Title: Ebola virus disrupts the inner blood-retinal barrier by induction of vascular endothelial growth factor in pericytes

doi: 10.1371/journal.ppat.1011077

Figure Lengend Snippet: (A) Western blot analysis of VLP-ΔGP using an anti-GP antibody. Representative images of three independent experiments are shown. (B) TEM image of VLP-ΔGP. (C) Permeability and (D) TEER of the tri-culture iBRB model were measured 48 h after VLP-ΔGP, VLP-ΔGP + Avastin and VEGF treatment. TEER values were normalized to those of iBRB models themselves before EBO-VLP administration. The box and the whisker present the median ± percentiles (25–75%) and range, respectively. The fold change of permeability compared with iBRB model itself before EBO-VLP administration is presented as the mean ± standard deviation. All values were determined in six independent experiments. (E) The expression of VEGF in HRP after VLP-ΔGP treatment, as quantified by ELISA. The horizontal dashed line marks the limit of detection of the assay. The results are presented as the means ± standard deviation of three independent experiments. (F) The expression of VEGF in HRP after EBOV VP40 and GP was expressed in HRPs. The horizontal dashed line marks the limit of detection of the assay. The results are presented as the means ± standard deviation of three independent experiments. Statistical analysis was performed using Student’s t test. *p < 0.05, **p < 0.01.

Article Snippet: The amount of VEGF in the supernatant was measured by a Human VEGF ELISA Kit (Boster, EK0539).

Techniques: Western Blot, Permeability, Whisker Assay, Standard Deviation, Expressing, Enzyme-linked Immunosorbent Assay

Journal: PLOS Pathogens

Article Title: Ebola virus disrupts the inner blood-retinal barrier by induction of vascular endothelial growth factor in pericytes

doi: 10.1371/journal.ppat.1011077

Figure Lengend Snippet: (A) Immunohistofluorescence analysis of EBO-VLPs in the retinal tissue using anti-GP antibodies (red). The white arrow indicates EBO-VLPs. Representative images of three independent experiments are shown. The fluorescent images were taken with a 60× magnification objective lens under a confocal microscope. (B) H&E staining of retinas from rats treated with Avastin and/or EBO-VLPs and VEGF. Black arrows indicate the pathological destruction of the retina. Magnification: ×20. Representative images of three independent experiments are shown. (C) Evans blue assay showing rat retinal permeability at 2 days after EBO-VLP injection. The results are presented as the means ± standard deviation of four independent experiments. (D) Immunohistofluorescent staining of retinal sections was performed to analyze VEGF (red). Nuclear staining with DAPI shows the retinal layers. Representative images of three independent experiments are shown. (E) Western blot analysis of claudin-1 expression in rat retinas, which was normalized against β-actin. Representative images of three independent experiments are shown. (F) Relative protein levels of claudin-1 were normalized to β-actin. The results are presented as the means ± standard deviation of three independent experiments. (G) Immunohistofluorescent staining of claudin-1 in retinal sections. Representative images of three independent experiments are shown. (H) H&E staining of retinas from rats treated with or without VLP-ΔGP. Magnification: ×20. Representative images of three independent experiments are shown. (I) Evans blue assay showing rat retinal permeability at 2 days after VLP-ΔGP injection. The results are presented as the means ± standard deviation of four independent experiments. (J) Images of immunofluorescence staining of claudin-1 in retinal sections treated with VLP-ΔGP. Representative images of three independent experiments are shown. Statistical analysis was performed using Student’s t test. *p < 0.05, **p < 0.01.

Article Snippet: The amount of VEGF in the supernatant was measured by a Human VEGF ELISA Kit (Boster, EK0539).

Techniques: Immunohistofluorescence, Microscopy, Staining, Evans Blue Assay, Permeability, Injection, Standard Deviation, Western Blot, Expressing, Immunofluorescence

Journal: PLOS Pathogens

Article Title: Ebola virus disrupts the inner blood-retinal barrier by induction of vascular endothelial growth factor in pericytes

doi: 10.1371/journal.ppat.1011077

Figure Lengend Snippet: EBOV stimulates pericytes to secrete VEGF to cause the iBRB breakdown. VEGF disrupts iBRB through downregulation of the tight junction protein claudin-1. The VEGF antibody Avastin can reduce the effect on VLP-induced iBRB breakdown.

Article Snippet: The amount of VEGF in the supernatant was measured by a Human VEGF ELISA Kit (Boster, EK0539).

Techniques:

Journal: Lung

Article Title: FLRT3 Overexpression Attenuates Ischemia–Reperfusion Induced Vascular Hyperpermeability and Lung Injury Through RND3

doi: 10.1007/s00408-025-00791-w

Figure Lengend Snippet: Overexpression of FLRT3 promotes cell migration and rescues H/R-induced cell death and inhibition on cell migration. HPMECs were transduced with LV-Control, LV-FLRT3, sh-Control or sh-FLRT3 for 48 h. A Western blot confirmed successful FLRT3 overexpression and knockdown in HPMECs. B CCK-8 assay showed FLRT3 manipulation did not affect baseline cell viability. C Wound healing assay (24 h) demonstrated enhanced migration in LV-FLRT3 group and reduced migration in sh-FLRT3 group. D Transwell migration assay validated the migration changes with FLRT3 manipulation. E Western blot showed LV-FLRT3 restored H/R-induced FLRT3 reduction. F CCK-8 assay revealed FLRT3 overexpression protected against H/R-induced viability decrease. G Wound healing assay demonstrated FLRT3 overexpression preserved migration capacity under H/R conditions. H Transwell assay confirmed the protective effect of FLRT3 on H/R-impaired cell migration. Data are mean ± SD ( n = 3/group). Statistical significance was determined using one-way ANOVA and Tukey’s multiple comparisons test to compare multiple groups. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with the indicated groups

Article Snippet: Human pulmonary microvascular endothelial cells (HPMECs), purchased from ATCC (Manassas, VA, USA), were cultured in endothelial cell medium (ECM, Gibco, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Gibco), 1% endothelial cell growth factor (Beyotime Biotechnology, Jiangsu, China), and 100 IU/ml penicillin and streptomycin (Sigma-Aldrich) at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Over Expression, Migration, Inhibition, Transduction, Control, Western Blot, Knockdown, CCK-8 Assay, Wound Healing Assay, Transwell Migration Assay, Transwell Assay

Journal: Lung

Article Title: FLRT3 Overexpression Attenuates Ischemia–Reperfusion Induced Vascular Hyperpermeability and Lung Injury Through RND3

doi: 10.1007/s00408-025-00791-w

Figure Lengend Snippet: FLRT3 overexpression increases endothelial barrier integrity via cytoskeletal remodeling and restoration of cell–cell junctions after H/R. HPMECs were transduced with LV-Control or LV-FLRT3 for 48 h, then subjected to H/R. A TEER measurements showed FLRT3 overexpression prevented H/R-induced barrier dysfunction. B Evans blue permeability assay demonstrated FLRT3 overexpression reduced H/R-induced barrier leakage. C F-actin staining (FITC-phalloidin, green; DAPI, blue) revealed FLRT3 overexpression maintained cytoskeletal organization under H/R. Scale bar, 50 μm. D Western blot showed FLRT3 overexpression preserved junction protein levels after H/R. E VE-cadherin immunofluorescence demonstrated maintained adherens junctions with FLRT3 overexpression during H/R. Scale bar, 50 μm. F Claudin-5 immunofluorescence showed preserved tight junction organization with FLRT3 overexpression after H/R. Data are mean ± SD ( n = 3/group). Statistical significance was determined using one-way ANOVA and Tukey’s multiple comparisons test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with the indicated groups

Article Snippet: Human pulmonary microvascular endothelial cells (HPMECs), purchased from ATCC (Manassas, VA, USA), were cultured in endothelial cell medium (ECM, Gibco, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Gibco), 1% endothelial cell growth factor (Beyotime Biotechnology, Jiangsu, China), and 100 IU/ml penicillin and streptomycin (Sigma-Aldrich) at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Over Expression, Transduction, Control, Permeability, Staining, Western Blot, Immunofluorescence

Journal: Lung

Article Title: FLRT3 Overexpression Attenuates Ischemia–Reperfusion Induced Vascular Hyperpermeability and Lung Injury Through RND3

doi: 10.1007/s00408-025-00791-w

Figure Lengend Snippet: The FLRT3 protein is destabilized through the autophagic–lysosomal pathway after H/R. A qRT-PCR revealed unchanged FLRT3 mRNA levels in HPMECs after H/R ( n = 3/group). B qRT-PCR showed stable FLRT3 mRNA levels in lung tissue after I/R ( n = 5/group). C , D Protein stability assay using CHX (20 μg/ml) demonstrated accelerated FLRT3 degradation in H/R-treated HPMECs, with significant reduction at 2 h post-CHX ( n = 3/group). E Western blot showed proteasome inhibitor MG132 (5 μM) failed to prevent H/R-induced FLRT3 degradation ( n = 3/group). F Lysosomal inhibitors NH 4 Cl (10 µM) and CQ (10 μM) restored FLRT3 levels in H/R-treated HPMECs. Western blot quantification showed significant restoration of FLRT3 levels with both inhibitors ( n = 3/group). G Autophagy inhibitor Baf-A1 (100 nM) increased both LC3-II/LC3-I ratio and FLRT3 levels under H/R conditions ( n = 3/group). Data are presented as mean ± SD. Statistical significance was determined using unpaired Student’s t test to compare two groups and one-way ANOVA and Tukey’s multiple comparisons test to compare multiple groups. Two‐way ANOVA with Tukey’s post hoc test was used to compare multiple groups with two categorical variables. ** P < 0.01 compared with the indicated groups. Baf-A1 Bafilomycin-A1, CHX cycloheximide, CQ chloroquine, NH 4 Cl ammonium chloride

Article Snippet: Human pulmonary microvascular endothelial cells (HPMECs), purchased from ATCC (Manassas, VA, USA), were cultured in endothelial cell medium (ECM, Gibco, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Gibco), 1% endothelial cell growth factor (Beyotime Biotechnology, Jiangsu, China), and 100 IU/ml penicillin and streptomycin (Sigma-Aldrich) at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Quantitative RT-PCR, Stability Assay, Western Blot

Journal: Lung

Article Title: FLRT3 Overexpression Attenuates Ischemia–Reperfusion Induced Vascular Hyperpermeability and Lung Injury Through RND3

doi: 10.1007/s00408-025-00791-w

Figure Lengend Snippet: RND3 is a target of FLRT3. A HumanBase Gene Networks analysis predicted functional correlation between FLRT3 and RND3 in lung tissue. B Co-IP assay demonstrated endogenous FLRT3–RND3 protein interaction in HPMECs, with input and IgG controls confirming specificity. C GST pulldown assay validated direct FLRT3-RND3 binding. D Double immunofluorescence (FLRT3-red, RND3-green) showed protein co-localization in HPMECs. Scale bar, 50 μm. E Western blot revealed FLRT3 overexpression increased RND3 levels while FLRT3 knockdown reduced RND3 expression. F Western blot showed FLRT3 overexpression restored H/R-induced RND3 reduction ( P < 0.01). Data are mean ± SD ( n = 3/group). Statistical significance was determined using one-way ANOVA and Tukey’s multiple comparisons test. * P < 0.05, ** P < 0.01 compared with the indicated groups

Article Snippet: Human pulmonary microvascular endothelial cells (HPMECs), purchased from ATCC (Manassas, VA, USA), were cultured in endothelial cell medium (ECM, Gibco, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Gibco), 1% endothelial cell growth factor (Beyotime Biotechnology, Jiangsu, China), and 100 IU/ml penicillin and streptomycin (Sigma-Aldrich) at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Functional Assay, Co-Immunoprecipitation Assay, GST Pulldown Assay, Binding Assay, Immunofluorescence, Western Blot, Over Expression, Knockdown, Expressing

Journal: Lung

Article Title: FLRT3 Overexpression Attenuates Ischemia–Reperfusion Induced Vascular Hyperpermeability and Lung Injury Through RND3

doi: 10.1007/s00408-025-00791-w

Figure Lengend Snippet: RND3 is required for FLRT3 overexpression related cytoprotective effects after H/R in HPMEC cells. H/R-induced HPMECs were transduced with LV-FLRT3 and/or sh-RND3. A Western blot showed that overexpression of FLRT3 led to an increase in RND3 protein expression, while knockdown of RND3 in FLRT3-overexpressing HPMECs resulted in a reduction in RND3 protein expression levels. B , C Western blot showed RND3 knockdown reversed FLRT3-mediated protein expression under H/R. D TEER measurements revealed RND3 knockdown compromised FLRT3’s barrier-protective effect. E Evans blue assay validated RND3’s requirement in FLRT3-mediated barrier protection. F F-actin staining (phalloidin, green; DAPI, blue) showed RND3 knockdown prevented FLRT3’s cytoskeletal preservation. Scale bar, 50 μm. G Western blot demonstrated RND3 knockdown reversed FLRT3’s protection of junction proteins (VE-cadherin, ZO-1, claudin-5, and occludin). H , I Immunofluorescence of VE-cadherin ( H ) and claudin-5 ( I ) confirmed RND3’s role in FLRT3-mediated junction maintenance. Scale bar, 50 μm. Data are mean ± SD ( n = 3/group). Statistical significance was determined using one-way ANOVA and Tukey’s multiple comparisons test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with the indicated groups

Article Snippet: Human pulmonary microvascular endothelial cells (HPMECs), purchased from ATCC (Manassas, VA, USA), were cultured in endothelial cell medium (ECM, Gibco, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Gibco), 1% endothelial cell growth factor (Beyotime Biotechnology, Jiangsu, China), and 100 IU/ml penicillin and streptomycin (Sigma-Aldrich) at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Over Expression, Transduction, Western Blot, Expressing, Knockdown, Evans Blue Assay, Staining, Preserving, Immunofluorescence

Journal: Memórias do Instituto Oswaldo Cruz

Article Title: Intravenous whole blood transfusion results in faster recovery of vascular integrity and increased survival in experimental cerebral malaria

doi: 10.1590/0074-02760220184

Figure Lengend Snippet: effect of intravenous whole blood transfusion plus artemether on plasma angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) profiles in mice with experimental cerebral malaria (ECM). Plasma levels of angiopoietins among studied groups (healthy controls, blood plus artemether “ARM” and artemether alone) were measured using enzyme-linked immunosorbent assays (ELISA) (n = 10 mice per group). Mice with ECM showed depleted plasma Ang-1 levels (A) compared to uninfected controls (p < 0.0001). Treatment of ECM mice with artemether had no effect on Ang-1 levels, which remained very low. However, Ang-1 levels were slightly (mean 43%) but significantly higher in ECM mice that received whole blood transfusion compared to mice that received artemether alone at 6 h (p = 0.0499) and at 24 h whole blood transfusion led to a 2-fold increase in Ang-1 levels compared to artemether alone (p < 0.0001). (B) Plasma Ang-2 levels increased by 1.5 fold in ECM mice compared to normal controls (p = 0.0001). Treatment with artemether alone or artemether plus whole blood transfusion did not modify the increased Ang-2 levels 6 h after treatment. At 24 h, ECM mice that received whole blood transfusion showed plasma Ang-2 levels back to normal, whereas ECM mice that received artemether alone still showed increased levels (p = 0.0462). (C) The ratio of Ang-2 to Ang-1 was increased 10-fold in ECM mice compared to healthy controls (p < 0.0001). The levels kept increasing after 6 h of artemether alone treatment but stabilized with artemether plus whole blood treatment (p = 0.0357). Within 24 h, Ang-2 levels were still high in ECM mice that received artemether only, but were much lower in ECM mice that received artemether plus whole blood transfusion (p = 0.0002). Data are shown as mean ± standard deviation. For comparing groups at a given timepoint, Student’s t-test was performed.

Article Snippet: Enzyme-linked immunosorbent assays (ELISA) for biochemical analysis of plasma components - To determine levels of Angiopoietin 1 & 2, Mouse Angiopoietin 1 & 2 Picokine ELISA kits (Boster) were used.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Memórias do Instituto Oswaldo Cruz

Article Title: Intravenous whole blood transfusion results in faster recovery of vascular integrity and increased survival in experimental cerebral malaria

doi: 10.1590/0074-02760220184

Figure Lengend Snippet: blood-brain barrier (BBB) permeability and plasma levels of haptoglobin in mice with experimental cerebral malaria (ECM) after treatment with artemether with or without intravenous blood transfusion. (A) The BBB permeability (Evans blue assay) in mice with ECM was increased 2.7-fold in relation to healthy controls (p < 0.0001). BBB permeability kept increasing (to 4.6-fold) 6 h after treatment with artemether (ARM) alone, but intravenous transfusion of whole blood prevented the further increase. Indeed, Evans blue leakage in the brain of transfused mice was less than half that observed in mice receiving artemether alone (p < 0.0001). At 24 h, BBB leakage decreased but was still evident in mice treated with artemether alone, whereas it returned to normal levels in mice that received artemether plus blood transfusion (p = 0.0126). (B) Haptoglobin levels in whole plasma was assessed using enzyme-linked immunosorbent assays (ELISA). Mice with ECM showed a mean 135% increase in plasma haptoglobin levels compared to uninfected controls (p < 0.0001). The levels of haptoglobin showed a decline in both treatment groups at 6 and 24 h. No difference between the treatments was noticed any time point. Data are shown as mean ± standard deviation. For comparing groups at a given timepoint, Student’s t-test was performed.

Article Snippet: Enzyme-linked immunosorbent assays (ELISA) for biochemical analysis of plasma components - To determine levels of Angiopoietin 1 & 2, Mouse Angiopoietin 1 & 2 Picokine ELISA kits (Boster) were used.

Techniques: Permeability, Clinical Proteomics, Evans Blue Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: List of ELISA tests, cell proliferation, and protein extraction kits used. For ELISA tests, dilution of the supernatants or cell lysate samples used and detection limits are also reported.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Enzyme-linked Immunosorbent Assay, Protein Extraction

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: C-Kit (CD117) mRNA (a, b, c) and protein (d, e, f) expression after ESW treatment in primary bronchial fibroblasts of COPD patients (a, d), primary bronchial fibroblasts of control smokers (b, e), and bronchial epithelial cells (c, f). In bronchial epithelium (16HBE) c-Kit increased at mRNA (c) and protein (f) levels. In primary bronchial fibroblasts of COPD patients, c-Kit increased at protein level (d). T -test was used for comparative purposes, and p values are reported in the graphs.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing thyroid transcription factor-1 (TTF-1) expression (panels a, b), c-Kit (CD117) (c, d), and proliferating cell nuclear antigen (PCNA) (e, f) in the peripheral lung tissue of a representative patient with chronic obstructive pulmonary disease (COPD). Arrows indicate positively stained cells mainly located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing, Staining

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing alveolar type II epithelial cells (TTF-1+ cells, red color) coexpressing c-Kit (CD117) (brown color) (a, b) and PCNA (brown color) (c, d) in the peripheral lung tissue of a representative patient with COPD. Positive double-stained cells can be recognized in the alveolar septa, even though their presence was only rarely observed. Arrows indicate positively stained cells located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Staining

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: Expression of CD4, CD8, CD20, CD56, CD68, CD117, and CD177 by immune cells infiltrated tumor tissues of GC patients. Representative images of immune markers staining in immune cells from GC samples are shown at ×200 (100 mm) original magnification. Positive expression of CD4 (a), CD8 (b), CD20 (c), CD56 (d), CD68 (e), CD117 (f), and CD177 (g) infiltrated in tumor tissues with a regular distribution. In order to show the distribution from immune cells more clearly, we used computerized imaging system Image-Pro Plus version 6.2 to highlight positive areas in different colors.

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques: Expressing, Staining, Imaging

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: The schematic diagram of the distribution of immune cells. The blue points represent CD20 + B cells, the yellow points represent CD4 + T cells, the orange points represent CD117 + mast cells, the green points represent CD68 + macrophages, the pink points represent CD8 + T cells, the black points represent CD56 + NK cells, and the red points represent CD177 + neutrophils. Although not all immune cells are distributed according to this fixed law, this pattern can basically reflect the general characteristics of their distribution.

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques:

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: Differences in immune marker positive area/total area between the two groups (survival time of patients in group A was less than 1 year and group B survival time was more than 5 years) by the rank sum test. (a) CD4 + T cells ( P < 0.001). (b) CD8 + T cells ( P < 0.001). (c) CD20 + B cells ( P < 0.001). (d) CD68 + macrophages ( P < 0.001). (e) CD117 + mast cells ( P < 0.001). (f) CD177 + neutrophils ( P < 0.001).

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques: Marker